| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Estes, Lulu F (6)
|
PVPM |
23.83 |
22.32 |
-1.51 |
| 2 |
Goudie, Elise C (6)
|
PVMACH |
21.97 |
23.42 |
1.45 |
| 3 |
Nguyen, Sloane M (5)
|
PVMACH |
22.96 |
23.97 |
1.01 |
| 4 |
van de Graaff, Alexis L (6)
|
PVMAKO |
23.91 |
24.91 |
1.00 |
| 5 |
Frantz, Graycen M (6)
|
PVMACH |
27.70 |
29.66 |
1.96 |
| 6 |
Jordan, Brianna R (6)
|
PVYORK |
39.27 |
34.71 |
-4.56 |
| 7 |
Mulholland, Brooklyn P (6)
|
PVPM |
33.78 |
35.83 |
2.05 |
| 8 |
Branham, Maeve E (6)
|
PVMACH |
38.66 |
40.54 |
1.88 |
|
Grover, Vachi (6)
|
PVMACH |
|
NS |
- |
|
Javierre-Carmona, Ines I (6)
|
PVMACH |
|
NS |
- |
|
Huff, Isabel K (6)
|
PVPM |
24.23 |
NS |
- |